SILK FIBROIN NANOPARTICLES REVEAL EFFICIENT DELIVERY OF 5-FU IN A HT-29 COLORECTAL ADENOCARCINOMA MODEL IN VITRO
IONUȚ CRISTIAN RADU 1, ARIANA HUDIȚĂ 2, CĂTĂLIN ZAHARIA 1, CAROLINA NEGREI 3, GEORGE TRAIAN ALEXANDRU BURCEA DRAGOMIROIU 4, DANIELA ELENA POPA 4, MARIETA COSTACHE 2, HORIA IOVU 1, MARA GEORGESCU 7*, OCTAV GINGHINĂ 5,6, BIANCA GĂLĂȚEANU 2
1Advanced Polymer Materials Group, Politehnica University of Bucharest, Bucharest, Romania
2Department of Biochemistry and Molecular Biology, University of Bucharest, Romania
3“Carol Davila” University of Medicine and Pharmacy, Faculty of Pharmacy, Department of Toxicology, Bucharest, Romania
4“Carol Davila” University of Medicine and Pharmacy, Faculty of Pharmacy, Department of Drug Control, Bucharest, Romania
5Department of Surgery, “Sf. Ioan” Clinical Emergency Hospital, Bucharest, Romania
6“Carol Davila” University of Medicine and Pharmacy, Faculty of Dental Medicine, Department II, Bucharest, Romania
7University of Agronomic Sciences and Veterinary Medicine of Bucharest, Faculty of Veterinary Medicine, 59 Mărăşti Boulevard, Bucharest, 011464, Romania
*corresponding author: dr_georgescu_mara@yahoo.com
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Abstract:
The aim of the paper was to investigate the efficiency of 5-FU delivery from smart silk fibroin nanoparticles in a HT-29 colorectal adenocarcinoma model in vitro. Therefore, nanoparticles with various amounts of silk fibroin were obtained by nanoprecipitation method and characterized to evaluate the 5-FU encapsulation efficiency and release profile. All nanoparticles showed a good entrapment of the drug and were capable to release 5-FU in a polymer concentration dependent manner. The in vitro biological evaluation revealed an excellent biocompatibility of the pristine silk fibroin nanocarriers, while 5-FU loaded nanoparticles proved to be highly cytotoxic and dramatically decreased the HT-29 cellular viability and proliferation potential. More, the 5-FU silk fibroin nanoparticles induced alterations in the HT-29 cellular morphology and inhibited the formation of compact cellular clusters.
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