CHEMICAL COMPOSITION AND ANTI-INFLAMMATORY ACTIVITY OF KITAIBELIA BALANSAE BOISS.
GÖKÇE ŞEKER KARATOPRAK 1*, LEYLA PAŞAYEVA 1, ESRA KÖNGÜL ŞAFAK 1, FATIH GÖGER 2, OSMAN TUGAY 3, MÜBERRA KOŞAR 4
1Department of Pharmacognosy, Faculty of Pharmacy, Erciyes University, Kayseri, Turkey
2Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, Eskişehir, Turkey
3Department of Botany, Faculty of Art and Science, Selçuk University, Konya, Turkey
4Department of Pharmacognosy, Faculty of Pharmacy, Eastern Mediterranean University, Famagusta, North Cyprus, via Mersin-10 Turkey
*corresponding author: gskaratoprak@gmail.com
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Abstract:
Aerial part extracts of Kitaibelia balansae Boiss. were examined for their phenolic contents, as well as anti-inflammatory activities. The chemical compositions of the extracts were analysed by spectrophotometric and chromatographic (HPLC/ MS/MS and HPLC-PDA) approaches. To measure anti-inflammatory activity, the effects of the extracts on nitric oxide (NO) and Tumour Necrosis Factor-α (TNF-α) levels were measured in lipopolysaccharide (LPS) treated murine macrophage cell line (RAW 264.7). The ethyl acetate fraction was found to be abundant in phenolic compounds (259.78 ± 15.07 mgGAE/ gextract). Rutin was identified as the major compound in all the extracts. The ethyl acetate fraction potentially inhibited NO and TNF-α production (37.26 µM and 1781.524 pg/mL, respectively, 6 h) in LPS induced cells.
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