HPTLC QUANTIFICATION OF POLYPHENOLIC ACIDS AND ANTIOXIDANT ACTIVITY OF POLYGONUM HYDROPIPER L. SPECIES FROM ROMANIAN FLORA
ROXANA KOSTICI 1, ANDREI BIŢĂ 2*, LUDOVIC-EVERARD BEJENARU 2, CORNELIA BEJENARU 3, FLORIN-DAN POPESCU 4, GEORGE DAN MOGOŞANU 2, FLORICA POPESCU 1
1Department of Pharmacology, Faculty of Medicine, University of Medicine and Pharmacy of Craiova, 2 Petru Rareş Street, 200349 Craiova, Dolj County, Romania
2Department of Pharmacognosy & Phytotherapy, Faculty of Pharmacy, University of Medicine and Pharmacy of Craiova, 2 Petru Rareş Street, 200349 Craiova, Dolj County, Romania
3Department of Pharmaceutical Botany, Faculty of Pharmacy, University of Medicine and Pharmacy of Craiova, 2 Petru Rareş Street, 200349 Craiova, Dolj County, Romania
4Department of Allergology, Faculty of Medicine, “Carol Davila” University of Medicine and Pharmacy, 8 Eroii Sanitari Avenue, District 5, 050474 Bucharest, Romania
*corresponding author: andreibita@gmail.com
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Abstract:
For the flowering aerial parts of Polygonum hydropiper L. species, harvested from Romanian flora, four polyphenolic acids were separated and quantified using HPTLC-UV densitometry, with ESI-MS confirmation. Chlorogenic acid was found in the highest amount, followed by ferulic acid, p-coumaric acid and caffeic acid. The content of total polyphenols and caffeic acid derivatives was determined using spectrophotometric methods. In vitro antioxidant activity was evaluated using DPPH assay. Our studies bring new data regarding the phytochemical profile of P. hydropiper, as a natural source of polyphenols with antioxidant properties.
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