LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY METHODS
FOR THE DETERMINATION OF ANDARINE AND OSTARINE IN RAT
SERUM AFTER PROTEIN PRECIPITATION
AMALIA MIKLOS 1, AMELIA TERO-VESCAN 1*, LENÁRD FARCZÁDI 2,3, SILVIA IMRE 1,2, CAMIL-EUGEN VARI 1, DANIELA-LUCIA MUNTEAN 1
1Faculty of Pharmacy, University of Medicine, Pharmacy, Sciences and Technology, 38 Gheorghe Marinescu Street, 540139,
Târgu Mureş, Romania
2Center for Advanced Medical and Pharmaceutical Research (CCAMF), University of Medicine, Pharmacy, Sciences and
Technology, 38 Gheorghe Marinescu Street, 540139, Târgu Mureş, Romania
3Faculty of Pharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy, 8 Victor Babeş Street, 400012, Cluj-
Napoca, Romania
*corresponding author: amelia.tero-vescan@umfst.ro
Download Full Article

Abstract:
Quantitative determination of andarine and ostarine in rat serum was achieved using two selective liquid chromatographymass
spectrometry methods (LC-MS). After extracting the analytes and internal standards (ostarine and andarine) from the
serum samples through a high throughput extraction method (protein precipitation), the samples were analysed by reverse
phase liquid chromatography using as mobile phase a mixture of methanol and 10 mM ammonium formate solution (75:25,
v/v). The analytes were detected following specific transitions in MS/MS mode of the QTOF mass spectrometer, after preionization
by a negative electrospray ionization source (ESI-). Both methods were validated for a concentration range
between 50 - 10000 ng/mL according to the current official guidelines with regards to carry-over, selectivity, linearity,
within- and between-run accuracy, precision and can be applied to accurately measure andarine and ostarine levels in rat
serum samples in order to design a pharmacokinetic study or for doping control purposes.

Top