CHEMICAL COMPOSITION AND ANTIOXIDANT ACTIVITY OF
FICUS ELASTICA ROXB. EX HORNEM AND RAPHANUS SATIVUS L.
SELECTIVE DRY EXTRACTS WITH POTENTIAL ANTIDIABETIC
ACTIVITY
AL HILFI ZAID ABDULRIDHA FLAYYH 1#, IOANA NENCU 2*, TEODORA COSTEA 2#, CERASELA ELENA GÎRD 2, CRISTINA SILVIA STOICESCU 3#, ADRIANA IULIANA ANGHEL 3#, ROBERT VIOREL ANCUCEANU 3#, MIHAELA DINU 3, FLORIANA ELVIRA IONICĂ 4, OANA CRISTINA ŞEREMET 1, SIMONA NEGREŞ 1
1Pharmacology and Clinical Pharmacy Department, Faculty of Pharmacy, “Carol Davila” University of Medicine and
Pharmacy, 6 Traian Vuia Street, 020956, Bucharest, Romania
2Pharmacognosy, Phytochemistry, Phytotherapy Department, Faculty of Pharmacy, “Carol Davila” University of Medicine
and Pharmacy, 6 Traian Vuia Street, 020956, Bucharest, Romania
3Pharmaceutical Botany Department, Faculty of Pharmacy, “Carol Davila” University of Medicine and Pharmacy, 6 Traian
Vuia Street, 020956, Bucharest, Romania
4Faculty of Pharmacy, University of Medicine and Pharmacy of Craiova, Craiova, Romania
*corresponding author: ioanaitudor@gmail.com
#Authors with equal contribution
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Abstract:
The aim of our study consisted in obtaining, phytochemical and antioxidant activity evaluation of two selective dry extracts
from the leaves of Ficus elastica Roxb. ex Hornem and Raphanus sativus L., with potential use in metabolic diseases. The
results of the phytochemical assessment of the two hydroethanolic dry extracts (the employed hydroethanolic mixture were:
70% ethanol (v/v) for Fici elastici folium and 50% ethanol (v/v) for Raphani sativi folium) indicated the following: Fici
elastici extractum (FEE) - 3.6140±0.4000 g% flavonoids expressed as hyperoside, 5.950 ± 0.3800 g% phenolcarboxylic acids
expressed as chlorogenic acid, 13.3299 ± 0.1583 g% total phenolic compounds expressed as tannic acid; 2. Raphani sativi
extractum (RSE) - 6.7300 ± 0.6600 g% flavonoid expressed as hyperoside, 3.1263 ± 0.1500 g% phenolcarboxylic acids
expressed as chlorogenic acid, 21.4175 ± 0.9454 g% total phenolic compounds expressed as tannic acid. The HPLC analysis
confirmed the presence of apigenin-7-glucoside in Raphani sativi extractum. Evaluation of the antiradicalar activity of the dry
extracts was determined by scavenger activity of free radicals, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azinobis-(3-ethylbenzothiazoline-
6-sulfonic acid) (ABTS•+) and by ferric reducing power method. The antioxidant activity was expressed as
EC50 mg/mL. The following results were obtained: EC50DPPH = 6.4166 ± 0.3329 mg/mL, EC50ABTS = 0.0768 ± 0.0020 mg/mL,
EC50ferric reducing power = 0.4027 ± 0.0016 mg/mL (for FEE); EC50DPPH = 7.5100 ± 0.1414 mg/mL, EC50ABTS = 0.2611 ± 0.1893
mg/mL, EC50ferric reducing power =1.3935 ± 0.0111 mg/mL (for RSE).

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