EFFECTS OF GINSENOSIDE Rg3 ON THE PROLIFERATION OF
GLIOMA CELLS AND NF-κB SIGNALLING PATHWAY
YINDONG MU 1, YANG LIU 2, YANKUN HAO 3, LEI YAN 1, JUN LIANG 4, JIANJIANG DONG 1*
1Department of Histology and Embryology, Mudanjiang Medical University, Mudanjiang 157011, China
2Office of Educational Administration, Mudanjiang Medical University, Mudanjiang 157011, China
3Department of Medical Function, Mudanjiang Medical University, Mudanjiang 157011, China
4Stem Cell Institute, Mudanjiang Medical University, Mudanjiang 157011, China
*corresponding author: dongjianjiangmdjmu@163.com
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Abstract:
The objective of this study was to assess the effects of Ginsenoside Rg3 on the proliferation of glioma cells and NF-κB
(nuclear factor kappa-light-chain-enhancer of activated B cells) signalling pathway and to provide a reliable reference for
clinical treatments. The glioma U251 cells were cultured and treated with Ginsenoside Rg3 at different concentrations (0, 20,
40, 80, and 160 μg/mL) for 24, 48, and 72 h, respectively. The morphological changes of U251 cells were observed before
and after treatment with Ginsenoside Rg3. Cell proliferation was detected by the MTT assay. The expression of P65 gene
promoter was detected by Dual-Luciferase Reporter Assay. Besides, the expressions of p65 and inhibitory kappa B (IκB)
were detected by Western blotting. U251 cells before Ginsenoside Rg3 treatment were structurally intact and after the
treatment started to present cell shrinkage and cellular debris that become more pronounced with the increase of the treatment
duration. The lower concentration of Ginsenoside Rg3 could promote the expression of the P65 gene promoter, while the
expression gradually decreased with the increase of Ginsenoside Rg3 concentration. The expression of IκBα protein
gradually increased with the increase of Ginsenoside Rg3 concentration. In conclusion, Ginsenoside Rg3 can effectively
inhibit the proliferative activity of U251 cells and the NF-κB signalling pathway and induce the apoptosis of tumour cells.






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