FISH-OIL PRECONDITIONING UP-REGULATES EXPRESSION OF
SPLENIC ARG1 POSITIVE M2 TYPE MACROPHAGES AND THE ARG1/INOS2 RATIO AFTER EXPERIMENTAL INDUCED TRANSIENT
CEREBRAL ISCHEMIA
EMŐKE HORVÁTH 1*, ALEX ORĂDAN 2, LIVIU CHIRIAC 3,4, MINODORA DOBREANU 5,6, ELŐD-ERNŐ NAGY 7, SEPTIMIU VOIDĂZAN 8, ROBERT BEREI 9, DANIELA LUCIA MUNTEAN 10, ADINA HUȚANU 5,6
1Department of Pathology, University of Medicine, Pharmacy, Science and Technology, 38 Gh. Marinescu Street, Târgu
Mureș, Romania
2Laboratory Animal Facility-Centre for Experimental Medicine, “Iuliu Hațieganu” University of Medicine and Pharmacy, 6
Louis Pasteur Street, Cluj-Napoca, Romania
3National Magnetic Resonance Centre, Faculty of Physics, “Babeș-Bolyai” University, 1 Mihail Kogălniceanu Street, Cluj-
Napoca, Romania
4Department of Molecular Sciences, Faculty of Medicine, “Iuliu Hațieganu” University of Medicine and Pharmacy, 6 Louis
Pasteur Street, Cluj-Napoca, Romania
5Centre for Advanced Medical and Pharmaceutical Research, University of Medicine, Pharmacy, Science and Technology,
38 Gh. Marinescu Street, Târgu Mureș, Romania
6Department of Laboratory Medicine, University of Medicine Pharmacy, Science and Technology, 38 Gh. Marinescu Street,
Târgu Mureș, Romania
7Department of Biochemistry, University of Medicine, Pharmacy, Science and Technology, 38 Gh. Marinescu Street, Târgu
Mureș, Romania
8Department of Epidemiology, University of Medicine, Pharmacy, Science and Technology, 38 Gh. Marinescu Street, Târgu
Mureș, Romania
9Faculty of General Medicine, University of Medicine, Pharmacy, Science and Technology, 38 Gh. Marinescu Street, Târgu
Mureș, Romania
10Department of Analytical Chemistry and Drug Analysis, University of Medicine, Pharmacy, Science and Technology, 38
Gh. Marinescu Street, Târgu Mureș, Romania
*corresponding author: emoke.horvath@umfst.ro
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Abstract:
During a stroke, the contraction of the spleen can mobilize inflammatory cells that infiltrate the damaged brain tissue. We
analysed the effects of transient cerebral ischemia and fish oil preconditioning on the spleen volume, architecture, and
macrophage polarization. Wistar rats were subjected to middle cerebral artery occlusion (ischemic and treatment groups, each
n = 10), and were sacrificed after 24 hrs, along with a sham group (n = 10). The spleen volume suffered only mild variations,
while the marginal zone showed size reduction in the ischemic group, compared to treated and control groups.
Granulopoiesis and myeloperoxidase positivity were significantly increased in spleens of ischemic animals (p < 0.001 vs. the
treatment and sham groups). Arginase 1 was up-regulated and the Arg1/iNOS2 expression ratio showed a significant shift on
macrophages of marginal zone/red pulp interface in the treatment group compared to ischemic rats (p < 0.001). Arg1 positive
M2 type monocytes/macrophages participate in resolution and tissue repair thus may feature as potential therapeutical targets
in cerebral ischemia.

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