ANTIBACTERIAL AND ANTIOXIDANT ACTIVITY OF
BIOSYNTHESIZED SILVER NANOPARTICLES FROM ULMUS
WALLICHIANA PLANCH. LEAF EXTRACT
ANSAR MEHMOOD 1*, SHAISTA SHABIR 1, SAJJAD HUSSAIN 1, KHAWAJA SHAFIQUE AHMAD 1, ABDUL HAMID 2
1Department of Botany, University of the Poonch Rawalakot, 12350, Azad Kashmir, Pakistan
2Department of Horticulture, University of the Poonch Rawalakot, 12350, Azad Kashmir, Pakistan
*corresponding author: ansar.mehmood321@gmail.com
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Abstract:
Eco-friendly synthesis of metal nanoparticles using plant extract plays a vital role in nanotechnology. This study aimed to produce
silver nanoparticles (SNPs) in an eco-friendly manner using leaf extract of Ulmus wallichiana Planch. When the leaf extract was
treated with AgNO3, the colour changed from yellowish to dark brown, indicated the formation of SNPs. The obtaining SNPs
were pre-dominantly spherical, crystalline and a mean size of 54 nm. FTIR spectrum evidenced that plant’s biomolecules acted
as reducing agent. The antibacterial activity of SNPs was assessed against Gram positive (Staphyllococcus aureus) and Gram
negative (Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae) bacteria and highest activity was found to
be exerted at 1 mg/mL SNPs against P. aeruginosa (17.63 mm). They also showed profound antioxidant activity against DPPH.
Ulmus mediated synthesis of SNPs is eco-friendly, non-toxic and cost effective with good antibacterial and antioxidant effect
and can be used in biomedical applications.

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