INVESTIGATION OF BIOLOGICAL ACTIVITIES AND SECONDARY
METABOLITES OF HYDNUM REPANDUM ACETONE EXTRACT
JOVANA TUBIĆ 1, DARKO GRUJIČIĆ 1, MARINA RADOVIĆ JAKOVLJEVIĆ 1, BRANISLAV RANKOVIĆ 1, MARIJANA KOSANIĆ 1, TATJANA STANOJKOVIĆ 2, ANDRIJA ĆIRIĆ 1, OLIVERA MILOŠEVIĆ-DJORDJEVIĆ 1,3*
1University of Kragujevac, Faculty of Science, 34000 Kragujevac, Serbia
2Institute of Oncology and Radiology of Serbia, 11000 Belgrade, Serbia
3University of Kragujevac, Faculty of Medical Sciences, 34000 Kragujevac, Serbia
*corresponding author: olivera@kg.ac.rs
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Abstract:
This study aimed to evaluate the biological activities and polyphenolic contents of the acetone extract of H. repandum
mushroom. Polyphenolic compounds were evaluated by high-performance liquid chromatography (HPLC). The antioxidant
activity was assessed by radical scavenging activity assays and reducing power. The antimicrobial activity was established
based on the values of the minimum inhibitory concentration (MIC) using microdilution method. Cytotoxic activity was
determined by 3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT). The genotoxic and antimutagenic
activities were tested using cytokinesis block micronucleus (CBMN) assay on human peripheral blood lymphocytes in vitro.
Among the determined polyphenolic compounds, ferulic acid and quercetin were mostly found. The extract showed high free
radical scavenging activity, while the reducing power was less emphasized and concentration-dependent. The MIC fluctuated
in a range of 0.009-10 mg/mL. The cytotoxic activity (based on IC50) ranged from 116.5 to 158.33 μg/mL, when HeLa cells
were the most sensitive. The highest tested concentrations of the extract showed significant genotoxic activity, while against
mitomycin C, the extract caused protective activity. The results indicated that H. repandum acetone extract contained
secondary metabolites which showed biological activities such as antioxidant, antimicrobial, cytotoxic, genotoxic and
protective against chemotherapeutics, indicating that their inclusion in nutrition could be of great importance in the
prevention and treatment of various pathological conditions.






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