COMPARATIVE PHYTOCHEMICAL AND ANTIOXIDATIVE
CHARACTERIZATION OF TRIFOLIUM PRATENSE L. AND OCIMUM
BASILICUM L.
INA-ANDREEA ANTONESCU (MINTAS) 1, TUNDE JURCA 2, FELICIA GLIGOR 3, IZABELA CRACIUN 3, LUMINITA FRITEA 1, EVA BRIGITTA PATAY 4, MARIANA MURESAN 1, DENISA IOANA UDEANU 5, CORINA ANA IONIȚĂ 5, ANGELA ANTONESCU 1*, FLORIAN BODOG 6
1University of Oradea, Faculty of Medicine and Pharmacy, Department of Preclinical Disciplines, Oradea, Romania
2University of Oradea, Faculty of Medicine and Pharmacy, Department of Pharmacy, Oradea, Romania
3University’’Lucian Blaga’’, Sibiu, Faculty of Medicine and Pharmacy, Department of Pharmacy, Sibiu, Romania
4University of Pecs, Faculty of Pharmacy, Department of Pharmacognosy, Pecs, Hungary
5University of Medicine and Pharmacy “Carol Davila”, Faculty of Pharmacy, Department of Clinical Laboratory and Food
Safety, Bucharest, Romania
6University of Oradea, Faculty of Medicine and Pharmacy, Department of Surgery, Oradea, Romania
*corresponding author: antonescu.angela@yahoo.com
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Abstract:
The phytochemical composition of Trifolium pratense L. and Ocimum basilicum L. was investigated by using
chromatographic and spectrophotometric methods. T. pratense extract was rich in ferulic acid (237.48mg/100g dry weight)
and chlorogenic acid (158.50 mg/100g dry weight), meanwhile O. basilicum extract presented the highest concentration in ferulic
acid (437.58 mg/100g dry weight). The determination of total phenolic and flavonoid contents indicated that O. basilicum
contained the highest amounts (67.89mg GAE/100g dry extract and 6.49 mg QE/mL extract respectively), aspect which was
corelated with its strongest antioxidant activity assessed by DPPH (2,2-diphenyl-1-picrylhydrazyl), FRAP (ferric reducing
antioxidant power) and CUPRAC (cupric reducing antioxidant capacity) methods.

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