ASSESSMENT OF LIPID PROFILE OF EIGHT PROPOLIS SAMPLES
FROM WESTERN ROMANIA
ALEXANDRA DUCA 1 , ERSILIA ALEXA 2, CRISTINA ADRIANA DEHELEAN 3, CODRUŢA ȘOICA 4, CORINA DANCIU 5, IULIANA POPESCU 6, ILEANA COCAN 7*, DACIAN LALESCU 7, DANINA MIRELA MUNTEAN 8
1Department of Physical Chemistry, Faculty of Pharmacy, “Victor Babeş” University of Medicine and Pharmacy, 2 Eftimie
Murgu Square, 300041, Timişoara, Romania
2Department of Food Control, Faculty of Agro-food Technologies, “Regele Mihai I al României” Banat’s University of
Agricultural Sciences and Veterinary Medicine, 119 Calea Aradului Street, 300641, Timişoara, Romania
3Department of Toxicology, Faculty of Pharmacy, “Victor Babeş” University of Medicine and Pharmacy, 2 Eftimie Murgu
Square, 300041, Timişoara, Romania
4Department of Pharmaceutical Chemistry, Faculty of Pharmacy, “Victor Babeş” University of Medicine and Pharmacy, 2
Eftimie Murgu Square, 300041, Timişoara, Romania
5Department of Pharmacognosy, Faculty of Pharmacy, “Victor Babeş” University of Medicine and Pharmacy, 2 Eftimie
Murgu Square, 30004, Timişoara, Romania
6Department of Soil Science, Faculty of Agriculture, “Regele Mihai I al României” Banat’s University of Agricultural
Sciences and Veterinary Medicine, 119 Calea Aradului Street, 300641, Timişoara, Romania
7Department of Food Technologies, Faculty of Agro-food Technologies, “Regele Mihai I al României” Banat’s University of
Agricultural Sciences and Veterinary Medicine, 119 Calea Aradului Street, 300641, Timişoara, Romania
8Department of Pathophysiology - Functional Sciences, Faculty of Medicine, "Victor Babeş" University of Medicine and
Pharmacy, 2 Eftimie Murgu Square, 300041, Timişoara, Romania
*corresponding author: negreaileana@yahoo.com
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Abstract:
Propolis is a natural resinous mixture produced by honeybees in order to strengthen, isolate and sanitize the hive that has
been systematically reported to exert a plethora of beneficial effects on human health. The aim of the present study was to
analyse the lipid profile of 8 propolis samples collected from producers in Western Romania. Lipids were extracted with a
Soxtest system and analysed by gas chromatography-mass spectrometry (GC-MS). The total lipid level showed an average
value of 33.17 ± 0.16% (w/w). A total number of 56 lipids were identified, among which ~ 30% were fatty acids, ranging
from 24.50% to 45.13% in lipids extracted; unsaturated fatty acids were reported between 4.22% and 13.40% in samples. In
particular, 8 fatty acids were identified as being present in all the investigated propolis samples: palmitic, oleic, behenic,
arachidic, stearic, α-linolenic (essential fatty acid from ω-3 series), myristic and lauric acid. Principal components analysis
and cluster analysis were performed to analyse the data.

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