EXPERIMENTAL AND MOLECULAR MODELING INVESTIGATION
OF THE REACTION BETWEEN CEFTRIAXONE AND SODIUM
NITRITE. POTENTIAL MEDICAL IMPLICATIONS
MARINELA FLOREA #*, CORINA CRISTINA ARAMĂ #, ANGELA NEDELCU #, IOANA CLEMENTINA CONSTANTINESCU #
Department of Analytical Chemistry, “Carol Davila” University of Medicine and Pharmacy, Faculty of Pharmacy, 6 Traian
Vuia Street, 020956, Bucharest, Romania
*corresponding author: florea.marinela@gmail.com
#Authors with equal contribution
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Abstract:
The paper presents a new spectrophotometric method for the assay of ceftriaxone as diazonium salt and the potential medical
implications of the reaction with sodium nitrite. Using KBr as catalyst, at 0 - 5°C and in acidic media a soluble and stable
compound with an absorption maximum at 504 nm was obtained. The method was linear in the range 85 - 171 μg/mL, with
limits of detection and quantification of 2.52 μg/mL and 7.65 μg/mL, respectively. For the diazonium ion, molecular
modelling indicated as stabilization mechanism an internal charge transfer and resonance light scattering spectra confirmed it.
The method is simple, rapid, accurate and precise, and successfully applied for the determination of ceftriaxone in
pharmaceutical products. Ceftriaxone administration can lead to nephrolithiasis, the high urinary antibiotic and calcium
concentrations being indicated to be the risk factors. The results of this study also indicated that the reaction of ceftriaxone
with sodium nitrite could also have potential medical implications in this regard.

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