PHENOLIC AND STEROLIC PROFILE OF A PHYLLANTHUS AMARUS
EXTRACT AND CHARACTERIZATION OF NEWLY SYNTHESIZED
SILVER NANOPARTICLES
ANDREIA CORCIOVĂ 1#, CORNELIA MIRCEA 1#*, CRISTINA TUCHILUŞ 2#, OANA CIOANCĂ 1#, ANA-FLAVIA BURLEC 1#, BIANCA IVĂNESCU 1#, LAURIAN VLASE 3#, ANA-MARIA GHELDIU 3#, ADRIAN FIFERE 4#, ANA-LĂCRĂMIOARA LUNGOCI 4#, MONICA HĂNCIANU 1#
1“Grigore T. Popa” University of Medicine and Pharmacy, Faculty of Pharmacy, 16 Universității Street, 700115, Iași, Romania
2“Grigore T. Popa” University of Medicine and Pharmacy, Faculty of Medicine, 16 Universității Street, 700115, Iași, Romania
3“Iuliu Hațieganu” University of Medicine and Pharmacy, Faculty of Pharmacy, 12 Ion Creangă Street, 400010, Cluj-Napoca,
Romania
4“Petru Poni” Institute of Macromolecular Chemistry, Center of Advanced Research in Bionanoconjugates and Biopolymers, 41A
Grigore Ghica-Vodă Alley, 700487, Iași, Romania
*corresponding author: corneliamircea@yahoo.com
#All authors have equal contribution
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Abstract:
The aim of this study was to identify and quantify some biologically active compounds from the Phyllanthus amarus species,
followed by the synthesis and characterization of silver nanoparticles. For identification and quantification purposes, different
methods were used, such as UV-Vis spectrophotometric methods and HPLC-UV-MS method for polyphenols analysis, LCMS
methods for methoxylated flavonoids and phytosterols analysis. After identification, the following compounds were
quantified: p-coumaric acid, ferulic acid, isoquercitrin, quercitrin, rutoside, hispidulin, acacetin, casticin, stigmasterol, betasitosterol,
and campesterol. To the best of our knowledge, this is the first report on the identification and quantification of
hispidulin, casticin, acacetin, and ergosterol in Phyllanthus amarus. Furthermore, the extract was used in order to obtain
silver nanoparticles (AgNPs), which were characterized by UV-Vis spectroscopy, FTIR, TEM, EDX. Additionally, the
nanoparticles were tested for antimicrobial and antioxidant activities. The results confirmed that Phyllanthus amarus is a
source of biological compounds that can be used for nanoparticles synthesis, with potential health use.






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