DETERMINATION OF SOME BIOGENIC AMINES IN RAT PLASMA
USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY TANDEM
MASS SPECTROMETRY (HPLC/MS) METHOD
NINA FILIP 1#, CORNELIA MIRCEA 2#*, CRISTINA IANCU 2#, MONICA HĂNCIANU 2#, ELENA COJOCARU 2#, BOGDAN STOICA 1#, CRISTIANA FILIP 1#
1“Grigore T. Popa” University of Medicine and Pharmacy, Faculty of Medicine, 16 University Street, 700115, Iași, Romania
2“Grigore T. Popa” University of Medicine and Pharmacy, Faculty of Pharmacy, 16 University Street, 700115, Iași, Romania
*corresponding author: corneliamircea@yahoo.com
# All authors have equal contribution
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Abstract:
Biogenic amines are compounds produced by human metabolism which, in small concentrations, exert physiological roles. In
high concentrations, biogenic amines can become toxic. In addition to the in vivo generation, biogenic amines result from
technological processes for the preparation and preservation of food products. These amines reach the body in concentrations
higher than normal, triggering severe side effects. Therefore, quantification of biogenic amines in both food and biological
fluids is of major interest. The aim of our study was to optimize and develop a modified HPLC/MS method for the
simultaneous separation and quantification of rat plasma histamine, tyramine, cadaverine and putrescine. The proposed
method is first applied for the determination of putrescine in rat plasma. The advantage of the method is the possibility of
quantifying trace amounts without the time-consuming phase of derivatization.

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