ANTIBACTERIAL ACTIVITY OF ETHANOLIC EXTRACTS FROM
FAGUS SYLVATICA L. AND JUGLANS REGIA L. LEAVES
ALINA IOANA NICU 1,2*, LUCIA PÎRVU 2, GHEORGHE STOIAN 3, ADRIAN VAMANU 1
1Faculty of Biotechnologies, University of Agronomic Science and Veterinary Medicine, 59 Marasti Boulevard, Bucharest,
Romania
2Pharmacology Department, National Institute for Chemical Pharmaceutical Research and Development – ICCF, 112 Vitan
Avenue, Bucharest, Romania
3Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 91-95 Splaiul
Independenței, Bucharest, Romania
*corresponding author: nicu_ioana@yahoo.com
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Abstract:
The use of medicinal plants is well known and modern science investigates the scientific bases for the effects of numerous plant
extracts against a great variety of diseases. Therefore, the aim of this study was to explore the antibacterial activity of the leaves
from two Romanian indigenous species: European beech (Fagus sylvatica L.) and walnut (Juglans regia L.). The leaves were
dried, then grinded and polyphenols were extracted using ethanol 70%. Afterwards, polyphenols and flavonoids were assayed
and identified accurately by HPTLC. Antimicrobial activity of the extracts was tested against several pathogenic bacteria
using the agar diffusion method and minimum inhibitory concentration determination. Our results showed a better activity
against Gram positive bacteria, and the most susceptible bacterial strain was Staphylococcus epidermidis.

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