ANTIMUTAGENIC AND ANTIMICROBIAL EFFECT OF AN
EXPERIMENTAL PLANT BASED EXTRACT FOR ENDODONTIC
USAGE
ANDRADA VOINA-ȚONEA 1*, MĂDĂLINA VĂLEANU 2, SORANA BACIU 1, MĂDĂLINA LAZĂR 1, CORINA VOINA 1, ANDREEA MIRON 1, DAN VODNAR 3
1“Iuliu Hațieganu” University of Medicine and Pharmacy, Faculty of Dental Medicine, Cluj-Napoca, Romania
2“Iuliu Hațieganu” University of Medicine and Pharmacy, Faculty of Medicine, Cluj-Napoca, Romania
3University of Agricultural Sciences and Veterinary Medicine, Faculty of Food Science and Technology, Cluj-Napoca, Romania
*corresponding author: andrada.tonea@umfcluj.ro
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Abstract:
An experimental extract based on Arctium lappa L. root powder and Aloe vera (L.) Burm.f. gel was developed for endodontic
usage. Its effect on the microbial viability was previously tested in vitro, against bacterial and fungal strains commonly found
in the human endodontic environment. The present study focuses on the in vivo evaluation of the antimicrobial effect of the
experimental plant based extract, using bacteria recovered from the infected root canals of the canine species. We determined
the number of colony-forming units before and after using the plant based product. The antimutagenic effect of the
experimental extract was determined by detecting mutagenic compounds with the help of the Salmonella mutagenicity test
(Ames test). Two auxotrophic reference strains were used, Salmonella typhimurium TA 98 and Salmonella typhimurium TA
100, which carried defective genes, related to the synthesis of histidine. Overall, results showed in vivo antimicrobial activity
of the experimental extract and also antimutagenic and inhibition effect against the two mutant bacterial strains.

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