HISTO-ANATOMICAL RESEARCHES, POLYPHENOLIC PROFILE
AND BIOLOGICAL ACTIVITIES OF THE AERIAL PARTS AND
TUBERS OF DACTYLORHIZA MACULATA L. SOÓ. (ORCHIDACEAE)
RAMONA PĂLTINEAN1#, IRINA IELCIU1#, LAURIAN VLASE2*, ANDREI MOCAN1, ANAMARIA GHELDIU2, MIHAI BABOTĂ1, DAN VODNAR3, GIANINA CRIȘAN1
1Department of Pharmaceutical Botany, Faculty of Pharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy,
Cluj-Napoca, Romania
2Department of Pharmaceutical Technology and Biopharmacy, Faculty of Pharmacy University of Medicine and Pharmacy
“Iuliu Hațieganu”, Cluj-Napoca, Romania
3Department of Food Science, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania
*corresponding author: laurian.vlase@umfcluj.ro
#These authors have equal contribution.
Download Full Article

Abstract:
The present study aimed to provide a histo-anatomical characterization, to evaluate the polyphenolic profile of the tubers and
aerial parts belonging to Dactylorhiza maculata L. Soó. and to test their antioxidant capacity and antibacterial effects.
Polyphenols were analysed by an HPLC-MS method. The antioxidant activity of the extracts was tested by TEAC and EPR
assays. Antibacterial activity was assessed on five bacterial strains. Ethanolic extracts of aerial parts proved to contain mainly
isoquercitrin, quercitrin, ferulic and p-coumaric acid, among the tested compounds. Assays of the biological activity revealed
a significant antioxidant and antibacterial activity especially for the aerial parts. At the same time, the histo-anatomical
features for the main constituents of aerial parts could be established. These results offer a new perspective on D. maculata,
proving it as an important source of bioactive compounds.

Top