IN VITRO IMPACT OF SOME NATURAL COMPOUNDS ON HT-29
COLORECTAL ADENOCARCINOMA CELLS
OCTAV GINGHINĂ1#, CAROLINA NEGREI2#*, ARIANA HUDIȚĂ3#, VLAD IOANA-LAVRIC3#, BIANCA GĂLĂȚEANU3#, SILVIA DRAGOMIR2#, GEORGE TRAIAN ALEXANDRU BURCEA DRAGOMIROIU4#, MARIA BÂRCĂ4#, CORNELIA NIȚIPIR5#, CAMELIA CRISTINA DIACONU6#,
ANCA-MIHAELA PANTEA STOIAN7#, NICULAE IORDACHE1#, ANDRA BĂLĂNESCU8#
1“Carol Davila” University of Medicine and Pharmacy, Faculty of Dental Medicine, Department of Surgery, “Sf. Ioan”
Clinical Emergency Hospital, 042122, Bucharest, Romania
2“Carol Davila” University of Medicine and Pharmacy, Faculty of Pharmacy, Departament of Toxicology, 020956, Bucharest,
Romania
3University of Bucharest, Faculty of Biology, Department of Biochemistry and Molecular Biology, 050095, Bucharest, Romania
4“Carol Davila” University of Medicine and Pharmacy, Faculty of Pharmacy, Departament of Drug Control, 020956,
Bucharest, Romania
5“Carol Davila” University of Medicine and Pharmacy, Faculty of Medicine, Department of Oncology, “Elias” Universitary
Emergency Hospital, 011461, Bucharest, Romania
6“Carol Davila” University of Medicine and Pharmacy, Faculty of Medicine, Department of Internal Medicine and
Gastroenterology, Clinical Emergency Hospital of Bucharest, 014461, Bucharest, Romania
7“Carol Davila” University of Medicine and Pharmacy, Department of Hygiene, 8 Eroii Sanitari Street, 050474, Bucharest,
Romania
8“Carol Davila” University of Medicine and Pharmacy, Faculty of Medicine, Department of Internal and Rheumatology,
”Sfânta Maria” Clinical Hospital, 011461, Bucharest, Romania
*corresponding author: carolina.negrei@outlook.com
#All authors had equal contribution to this research.
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Abstract:
Relatively recent research has robustly proved the role of phytochemicals present in vegetable and whole grain diets, fruit,
spices and various herbal teas in approach of cancer related risk. In recent times, this has been further supplemented with
research regarding the pharmacological effects of herbal medicinal products from the chemo-preventive perspective. The aim
of our study was to evaluate the potential effects that the association of silymarin and capsaicin could induce in HT-29
colorectal adenocarcinoma cells. For this, we treated HT-29 colorectal adenocarcinoma cells with silymarin, capsaicin and
with a combination of both compounds to investigate the cell viability, cell morphology as well as the DNA fragmentation as
a result of apoptosis. We found that both silymarin and capsaicin alone significantly decreased cell viability, determined
morphological changes and induced apoptosis. However, when used in combination, they mutually cancelled out their toxic
effects on HT-29 cells.

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