THE CORRELATION BETWEEN DIFFERENT DOSES OF GLUTAMINE
ON THE APOPTOSIS OF LYMPHOCYTES IN A MURINE MODEL OF
SEPSIS
RAN LIU1#, XIANKE LI1#, KAI YU1*, YONG WANG2
1Department of Anaesthesiology, the First People's Hospital of Jining, Jining, Shandong, 272011, China
2Anaesthesia Department of the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, 450052, China
*corresponding author: yu_kai123@sina.com
#The first two authors contributed equally to this work.
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Abstract:
The aim of this study was to investigate the effects of glutamine in different doses on the apoptosis of lymphocytes in septic
mice. 100 healthy mice were randomly divided into a control group (A), a sepsis model group (B), a high-dose glutamine
sepsis group (C, 0.75 mg/kgbw), a medium-dose glutamine sepsis group (D, 0.5 mg/kgbw) and a low-dose glutamine sepsis
group (E, 0.35 mg/kgbw), 20 animals per group. Mice from B, C, D and E groups underwent cecal ligation and puncture
(CLP), and then they were transformed into sepsis models. 24 hours after injection, the respiration, heart rate and rectal
temperature of the mice were recorded. The thymus and spleen specimens were taken and fixed. The apoptosis of
lymphocytes was detected by means of in suit end-labelling. Compared to the mice in group A, the mice in group B, C, D and
E had lower rectal temperature, increased respiratory frequency and increased heart rate, satisfying the diagnostic criteria for
animal models of Systemic Inflammatory Response Syndrome (SIRS). There were few apoptotic thymus and liver cells in
group A, while cell apoptosis was obvious in the other four groups, suggesting a significant difference compared to group A
(p < 0.05). Comparing the groups of different doses of glutamine, the number of apoptotic cells in groups C, D and E was
significantly lesser than that in group B (p < 0.05). Glutamine in different doses had a large impact on the apoptosis of
lymphocytes. The supplementation with glutamine can enhance the anti-apoptosis mechanism of the organism.






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