OPTIMIZED AND VALIDATED SPECTROPHOTOMETRIC
DETERMINATION OF BUTAMIRATE CITRATE IN BULK AND
DOSAGE FORMS USING ION-PAIR FORMATION WITH METHYL
ORANGE AND BROMOTHYMOL BLUE
DUYGU TAŞKIN1*, GIZEM ERENSOY2, SIDIKA SUNGUR1
1Istanbul Yeni Yuzyil University, Faculty of Pharmacy, Department of Analytical Chemistry, Istanbul, Turkey
2Istanbul Yeni Yuzyil University, Faculty of Pharmacy, Department of Pharmaceutical Chemistry, Istanbul, Turkey
*corresponding author: duygu.susuz@yeniyuzyil.edu.tr
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Abstract:
Two easy, sensitive, precise and valid spectrophotometric techniques are described for the determination of butamirate citrate (BC)
in pure form and pharmaceutical formulations. These methods are based on the formation of ion–pair between butamirate
citrate with methyl orange (MO) and bromothymol blue (BTB). The techniques involve the formation of yellow coloured ionpair
in aqueous solution, extraction with dichloromethane and absorbance measurement at 424 nm for BC-MO and 410 nm for
BC-BTB ion pairs against a blank solution. Molar ratio of the ion-pairs was determined using Job’s Continuous Variation Method.
The proposed methods were validated for linearity, limit of detection (LOD), limit of quantification (LOQ), accuracy and
precision. Beer’s law was obeyed in the ranges of 4.0 - 20.0 μg/mL for BTB and 2.5 - 12.5 μg/mL for MO.

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