DRY ROSE PETAL EXTRACTS AND COMPRESSED LOZENGES:
FORMULATION AND NIR – CHEMOMETRIC QUANTIFICATION OF
ACTIVE PRINCIPLES
ALEXANDRU GAVAN1*, ANCA MARIA TOIU2, MIRCEA TAMAȘ3, IOAN TOMUȚĂ1
“Iuliu Hațieganu” University of Medicine and Pharmacy, Faculty of Pharmacy, Cluj-Napoca, Romania
1Department of Pharmaceutical Technology and Biopharmaceutics,
2Department of Pharmacognosy,
3Department of Pharmaceutical Botany
*corresponding author: gavan.alexandru@umfcluj.ro
Download Full Article

Abstract:
The purpose of this study was to develop a manufacturing method for dry rose petal extracts and compressed tablet lozenges
and a new non-invasive near-infrared (NIR) – chemometric method for the quantification of their total polyphenolic content.
Formulations with four different concentrations were obtained through pulverization of Rosa damascena Mill alcoholic
extracts on solid isomalt support. The obtained dry extracts were further compressed into tablet lozenges. The total
polyphenolic content, expressed in gallic acid, was determined in all samples using the Folin-Ciocalteu reference method and
the results were used to calibrate the new developed quantification technique. The best results for the NIR – chemometric
technique were obtained by calculating the first derivative of the spectral values and then applying Multiple Scatter
Correction (MSC) or Standard Normal Variate (SNV) pre-processing techniques. In this way, the recovery percent of the
active principle content was between 99% and 101% for both products, dry extracts and compressed tablet lozenges. In
conclusion, high quality dry extracts and compressed lozenges have been developed and the active principles content could
be accurately quantified from the pharmaceutical products using NIR spectroscopy and chemometrics.

Top