POLYGONUM BISTORTA L. HERBA ET FLORES. POLYPHENOLS
PROFILE, ANTIOXIDANT PROPERTIES AND CYTOTOXIC EFFECT
ON MURINE FIBROBLAST CELL LINE NIH3T3
LUCIA PÎRVU1, FAWZIA SHA’AT2,3*, LUMINIȚA CLAUDIA MICLEA2, TUDOR SAVOPOL2, GEORGETA NEAGU1, DENISA IOANA UDEANU4, MIHAELA GEORGETA MOISESCU2
1National Institute for Chemical - Pharmaceutical Research and Development (INCDCF), 112 Vitan Road, 031299,
Bucharest, Romania
2“Carol Davila” University of Medicine and Pharmacy, Faculty of Medicine, Biophysics and Cellular Biotechnology
Department, 8 Eroii Sanitari Boulevard, 050474, Bucharest, Romania
3Politehnica University of Bucharest, Faculty of Applied Chemistry and Material Science, Analytical Chemistry and
Environmental Engineering Department, 1-7 Gheorghe Polizu Street, 011061, Bucharest, Romania
4“Carol Davila” University of Medicine and Pharmacy, Faculty of Pharmacy, Department of Clinical Laboratory and Food
Safety, 6 Traian Vuia Street, 020956, Bucharest, Romania
*corresponding author: fawzya.shaat@gmail.com
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Abstract:
This work aimed to study the polyphenols content, the antioxidant activity and the potential cytotoxic effects on fibroblast
murine cells NIH3T3 of an ethanolic extract (70%, v/v) obtained from the aerial part (herba et flores) of Polygonum bistorta
L. (Polygonaceae family). The tested extract was assessed for its total phenols content: 1.15 mg gallic acid equivalents
(GAE) per 1 mL extract and showed numerous flavonoids compounds such as quercetin, kaempferol and luteolin derivates,
as well as caffeic and chlorogenic phenolic acids. The chemiluminescence (CL) tests indicated a high antioxidant capacity
(IC50 = 0.57 μg GAE/mL), superior to gallic acid (IC50 = 0.85 μg/mL) and rutin (IC50 = 2.54 μg/mL), used as reference
compounds. The cytotoxicity test demonstrated that, at concentrations over 25 μg GAE/mL extract, the ethanolic extracts
from the aerial part of Polygonum bistorta plant species have a significant toxicity on mouse embryonic fibroblast NIH3T3 cells.

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