LC-MS/MS ESI METHODS FOR THE DETERMINATION OF
OESTROGENS AND ANDROGENS IN BIOLOGICAL MATRIX – A
MINIREVIEW
IBOLYA FÜLÖP, CAMIL-EUGEN VARI*, AMALIA MIKLOS, SILVIA IMRE
Faculty of Pharmacy, University of Medicine and Pharmacy Tîrgu Mureş, 38 Gheorghe Marinescu Street, 540139, Târgu
Mureş, Romania
*corresponding author: camil.vari@umftgm.ro
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Abstract:
This paper presents some of the determination methods of oestrogens (oestrone, oestradiol and oestriol) and androgens
(testosterone and 15α-testosterone) in biological matrix, highlighting the mass spectrometry (MS) technique. In clinical
laboratories, hormone measurements are based on immunoassay or liquid chromatography (LC) coupled with MS. Due to the
diversity of methods, the limits of normal values of oestrogens and androgens vary, making the interpretation of results
difficult. The scientific literature data shows that the LC-MS methods are the best choice for quantitative determination of
low levels of hormones. The most common ionization technique used for LC-MS analysis is electrospray ionization (ESI).
Sample preparation techniques, LC conditions, and MS parameters are revealed in order to compare the performances (limits
of detection and quantification) of different LC-ESI-MS/MS methods.

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