DNA CLEAVAGE STUDY AND SOD-MIMETIC ACTIVITY OF A NEW
Cu(II) COMPLEX
ADRIANA CORINA HANGAN1, ROXANA LIANA STAN2*, BOGDAN SEVASTRE3, SÎNZIANA GHEORGHE-CETEAN1, LUMINIȚA OPREAN1
1“Iuliu Hațieganu” University of Medicine and Pharmacy, Faculty of Pharmacy, Department of Inorganic Chemistry, 12 Ion
Creangă Street, 400010, Cluj-Napoca, Romania
2“Iuliu Hațieganu” University of Medicine and Pharmacy, Faculty of Pharmacy Department of Pharmaceutical Biochemistry
and Clinical Laboratory, 6 Pasteur Street, 400012, Cluj-Napoca, Romania
3University of Agricultural Science and Veterinary Medicine, Faculty of Veterinary Medicine, Paraclinic Department, 3-5
Calea Mănăștur Street, 400372, Cluj-Napoca, Romania
*corresponding author: roxanaluc@yahoo.com
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Abstract:
The DNA cleavage capacity of a new Cu(II) complex with substituted N-sulphonamide ligand, [Cu(L)2(phen)(H2O)] (HL = N-
(5-ethyl-[1,3,4] thiadiazole-2-yl)-benzenesulphonamide) was studied by analysing the process of damaging the DNA
molecule (pUC18 plasmid) in the presence of a reducing agent. Also, we tried to elucidate the reaction mechanism through
which the complex damages the DNA molecule by studying the free radicals and ions involved in the degradation of the
nucleic acid structure. The use of ROS (reactive oxygen species) scavengers indicates that the hydroxyl radicals and the
superoxide radical anions are the main species capable of breaking the DNA strands by an oxidative mechanism. The SODlike
activity of the complex was tested by an indirect method using the xanthine/xanthine oxidase system as source for
superoxide radicals. By this way, we demonstrated the SOD mimetic activity of the complex.

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