EVALUATION OF THE ANTIOXIDANT AND ANTIBACTERIAL
POTENTIAL OF BIOACTIVE COMPOUNDS FROM AJUGA REPTANS
EXTRACTS
ANCA TOIU1, LAURIAN VLASE2*, ANA MARIA GHELDIU2, DAN VODNAR3, ILIOARA ONIGA1
1Department of Pharmacognosy, Faculty of Pharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy, 8 V. Babeș
Street, Cluj-Napoca, Romania
2Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Pharmacy, “Iuliu Hațieganu” University of
Medicine and Pharmacy, 8, V. Babeș Street, Cluj-Napoca, Romania
3Department of Food Science, Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary
Medicine, 3-5 Mănăştur Street, Cluj-Napoca, Romania
*corresponding author: laurian.vlase@yahoo.com
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Abstract:
The aim of this study was to evaluate the polyphenols and iridoids from Ajuga reptans (Lamiaceae) flower extracts, and their
antioxidant and antibacterial activities. Total phenolic content (TPC), total flavonoid content (TFC), total anthocyanin
content (TAC) and HPLC/UV/MS were employed for the identification and quantification of polyphenolic compounds. Total
iridoid contents (TIC) were determined through a colorimetric method. The bioactive compounds were found in higher
quantities in the ethanol extracts. Caffeic, p-coumaric and ferulic acids, quercitrin, luteolin and apigenin were identified by
LC/MS in all samples. The antioxidant effects were evaluated by DPPH assay and TPC. A. reptans ethanol extract exhibited
a higher antioxidant activity than the methanol extract, and a positive correlation between the antioxidant properties and the
concentration of polyphenols was observed. The antimicrobial activity was evaluated by the use of dilution assays; the
minimal inhibitory concentration and the minimal bactericidal concentration values were determined. These results indicate
that A. reptans flowers contain active compounds with antioxidant and antibacterial potential.

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