POLYPHENOLIC PROFILE AND ANTIOXIDANT AND
ANTIBACTERIAL ACTIVITIES FROM TWO TRIFOLIUM SPECIES
DANIELA HANGANU1, DANIELA BENEDEC1*, LAURIAN VLASE2, NELI OLAH3, GRIGORE DAMIAN4, RADU SILAGHI-DUMITRESCU4, AUGUSTIN C. MOȚ4, CLAUDIA CRINA TOMA5
1Department of Pharmacognosy, “Iuliu Hațieganu” University of Medicine and Pharmacy, 12 I. Creangă Street, Cluj-
Napoca, Romania
2Department of Pharmaceutical Botany, “Iuliu Hațieganu” University of Medicine and Pharmacy, 12 I. Creangă Street,
Cluj-Napoca, Romania
3Department of Therapeutical Chemistry, Pharmaceutical Industry and Pharmaceutical Biotechnologies, “Vasile Goldiș”
Western University, 86 L. Rebreanu Street, Arad, Romania
4Department of Chemistry and Chemical Engineering, “Babeş-Bolyai” University, 11 A. Janos Street, Cluj-Napoca, Romania
5Department of Pharmacognosy, “Vasile Goldiș” Western University, 86 Rebreanu Street, Arad, Romania
*corresponding author: dani_67ro@yahoo.com
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Abstract:
In the present study, flowers and leaves of Trifolium pratense and T. repens were screened for evaluating the polyphenolic
contents and the antioxidant and antibacterial activities. A characterization of the main phenolic compounds was carried out
using HPLC-MS method. The total polyphenolic and flavonoid content was spectrophotometrically determined. Antioxidant
activity was evaluated by using several methods: DPPH (2,2-diphenyl-1-picrylhydrazyl) assay, TEAC (Trolox equivalent
antioxidant capacity), HAPX (haemoglobin ascorbate peroxidase activity inhibition) and EPR (electron paramagnetic
resonance) method. The polyphenolic profile revealed the presence of common components as p-coumaric acid, ferulic acid,
hyperoside, quercetin, luteolin, and other that make the differences (miricetol, quercitrin, isoquercitrin). T. pratense showed
better antioxidant and antibacterial activity than T. repens. The quantitative and qualitative differences between the two
species of Trifolium could be used as a potential taxonomic marker in order to distinguish the species.

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