RESVERATROL SUPPRESSED LPS-INDUCED COX-2 VIA miR-146a-5p
INHIBITION IN Raw246.7 CELLS
TRONG TUAN DAO1#, DO THI HA1#, TRAN THI HIEN2,3*, BUI THI BINH2, TRAN THI PHUONG4, PHUNG THANH LONG1,6, NGUYEN BICH THU1, DO THI NGUYET QUE5, NGUYEN MINH KHOI1, LE VIET DUNG1, TRAN MINH NGOC1
1National Institute of Medicinal Materials, 3B Quangtrung, Hanoi, Vietnam
2Department of Pharmacy, Thai Binh Medical and Pharmacy University, Vietnam
3Faculty of Medicine, Lund University, Sweden
4College of Pharmacy, Chonnam National University, South of Korea
5Hanoi University of Pharmacy, 13-15 Le Thanh Tong, Hanoi, Vietnam
6Dai Nam University, Vietnam
*corresponding author: thi.hien_tran@med.lu.se
#The first two authors contributed equally to this work.
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Abstract:
Trans-resveratrol (Res) is a well-known natural stilbene frequently found in grapes which have been reported to possess
antioxidant, anti-cancer activities and inhibited COX-2 expression. MicroRNAs (miRNAs) are short endogenous non-coding
RNAs involved in the regulation of mRNA stability and protein synthesis. In our research, resveratrol isolated from Vitis
heyneana Roem. & Schult Vitis heyneana was observed to suppress lipopolysaccharides (LPS)-induced COX-2 expression in
Raw264.7 cells in a dose dependent manner. Using qPCR it was revealed that LPS induced the expression of miR-25, miR-
125a, miR-125b, miR-146a-5p, miR-146a-3p and miR-455. However, we only observed miR-146a-5p expression
significantly decreased in resveratrol compared to untreated-control group. In addition, resveratrol abrogated the effect of
miR-146a-5p mimic induced-COX-2 expression in Raw264.7 cells. Taken together, this study demonstrated for the first time
the involvement of miR-146a-5p in resveratrol inhibited LPS-induced COX-2 expression in Raw264.7 cells.

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