CHEMICAL, ANTIOXIDANT AND ANTIBACTERIAL STUDIES OF
ROMANIAN HERACLEUM SPHONDYLIUM
DANIELA BENEDEC1#, DANIELA HANGANU1#, LORENA FILIP1#, ILIOARA ONIGA1*, BRÎNDUȘA TIPERCIUC1#, NELI-KINGA OLAH2#, ANA-MARIA GHELDIU1, OANA RAITA3, LAURIAN VLASE1
1“Iuliu Hațieganu” University of Medicine and Pharmacy, 8 V. Babeș Street, Cluj-Napoca, Romania
2“Vasile Goldiș” Western University of Arad, Faculty of Pharmacy, 86 L. Rebreanu Street, Arad and SC PlantExtrakt SRL,
Rădaia, Cluj, Romania
3National Institute for Research and Development of Isotopic and Molecular Technologies, 67-103 Donath Street, Cluj-
Napoca, Romania
*corresponding author: ilioara4@yahoo.com
#These authors contributed equally to this work.
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Abstract:
In order to obtain a chemical and biological characterization of Heracleum sphondylium subsp. sphondylium, we proposed
the qualitative and quantitative analysis of polyphenolic compounds from roots, stems, leaves, flowers and fruits, as well as
the antioxidant and antibacterial potential evaluation. For the identification and quantification of the phenolic compounds,
chromatographic and spectrophotometric methods were employed. The antioxidant activity was evaluated using DPPH (2,2-
diphenyl-1-picrylhydrazyl) radical scavenging, FRAP (ferric reducing antioxidant power) and EPR (electron paramagnetic
resonance) methods. The antibacterial test was performed by means of agar diffusimetric method. The HPLC (high
performance liquid chromatography) phenolic profile has revealed high amounts of rutin in flowers (983.88 mg/100 g) and in
leaves (477.08 mg/100 g), other flavonoids: quercitrin (15.60 mg/100 g leaves), quercetin (13.38 mg/100 g flowers) and
phenol acids: ferulic acid (13.04 mg/100 g) and chlorogenic acid (4.32 mg/100 g) in roots. The flowers and leaves extracts
showed the highest antioxidant capacity, according to the phenolic content. The antimicrobial tests revealed a good inhibitory
activity against S. aureus and L. monocytogenes for all samples. The results of the present investigation showed both
qualitative and quantitative differences between the parts of H. sphondylium, so it is recommended to use in practice the
extract obtained from a specific organ that furnishes the higher yield of active constituents.






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