NEW LC-MS/MS METHOD FOR TESTOSTERONE AND
DIHYDROTESTOSTERONE ANALYSIS IN MALE RAT PLASMA
LAURIAN VLASE1, SILVIA IMRE2*, DANIELA-LUCIA MUNTEAN2, BIANCA ŐSZ2, AMALIA MIKLOS2, AMELIA TERO-VESCAN2, CAMIL-EUGEN VARI2
1Faculty of Pharmacy, “Iuliu Haţieganu” University of Medicine and Pharmacy, 41 Victor Babeş Street, 400012, Cluj-Napoca, Romania
2Faculty of Pharmacy, University of Medicine and Pharmacy, 39 Gheorghe Marinescu Street, 540139, Târgu Mureş, Romania
*corresponding author: silsta@yahoo.com
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Abstract:
A new LC-MS/MS method for testosterone and dihydrotestosterone determination in male rat plasma is proposed. After a
simple protein precipitation, the compounds were separated on a C18 column with 1 mM ammonium formate solution and
methanol in gradient elution at 40°C and detected by an ion trap mass spectrometer after positive electrospray ionisation. The
monitoring ions were m/z 97.2, 109.2, 253.2 for testosterone and m/z 255.2 for dihydrotestosterone. The linearity domain
was established between 1.0 and 64 ng/mL for both compounds. The ranges of values for within- and inter-day accuracy and
precision were 89 - 111% and 2 - 13%, respectively. The proposed method is suitable for research purpose such as studies
regarding the influence of aromatase inhibitors on male rat plasma levels of testosterone and dihydrotestosterone.






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