EFFECT OF PLATELETS ANTIAGGREGANT CLOPIDOGREL ON
ERYTHROCYTES AGGREGATION AND SEDIMENTATION
STILIYANA BORISOVA1, IULIAN SARBU1, SIMONA NICOLETA SAVU1,2, ERAND MATI1*
1“Carol Davila” University of Medicine and Pharmacy, Faculty of Pharmacy, Doctoral School, Bucharest, Romania
2 Pharma Serv Int’l SRL, 52 Sabinelor Str., 5th District, 050853 Bucharest, Romania
*corresponding author: mail@em.al
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Abstract:
Based on theoretical biophysical considerations (Einstein - Stokes and extended Derjaguin, Landau, Verwey and Overbeeck
theories), it was predicted that platelet antiaggregant clopidogrel has to reduce the aggregation and sedimentation of
erythrocytes. Clopidogrel solutions were added to blood samples collected on EDTA for obtaining concentrations within the range
1 - 8 μg/mL, which equals the total plasma concentration of clopidogrel and its metabolites. Sedimentation curves were
obtained using the standard Westergren method. For each curve, the starting time, the slope of the linear part and the final
height of sediment were evaluated. The comparison of sedimentation curves using f2 and area under curve (AUC) metrics
from bio-pharmacy was an alternative approach to statistical tests, considered more appropriate for clinical interpretation.
The dependence between local structure of curves, as well as the area under sedimentation curve (AUSC) global parameter
on the concentration of clopidogrel was, as a rule, linear within the range 1 - 4 μg/mL clopidogrel concentration. As a final
conclusion, authors consider that results justify the hope that sedimentation of erythrocytes could become an alternative, ex
vivo, simple method for screening estimation of the extent of clopidogrel therapeutic effect.

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