POLYPHENOLIC CONTENT AND ANTIOXIDANT ACTIVITY OF
CHRYSANTHEMUM PARTHENIUM EXTRACT
DANIELA HANGANU1#, DANIELA BENEDEC1#, LAURIAN VLASE1*, IULIA POPICA1, CONSTANTIN BELE2, OANA RAITA3, ANA-MARIA GHELDIU1, CIPRIAN VALENTIN MIHALI4, VIORICA ȚĂRMURE1
1“Iuliu Hatieganu” University of Medicine and Pharmacy, 8 V. Babes Street, Cluj-Napoca, Romania
2University of Agricultural Sciences and Veterinary Medicine, 3-5 Mănăştur Street, Cluj-Napoca, Romania
3National Institute for Research and Development of Isotopic and Molecular Technologies, 65-103 Donath Street, Cluj-
Napoca, Romania
4Institute of Life Sciences, “Vasile Goldiş” Western University, 86 L. Rebreanu Street, Arad, Romania
*corresponding author: laurian.vlase@yahoo.com
#These authors contributed equally to this work.
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Abstract:
The main objective of this study was the phytochemical and biological analysis of the polyphenolic compounds of
Chrysanthemum parthenium. The phenolic compounds were analysed using the reference high performance liquid
chromatography (HPLC) method. The evaluation of the polyphenolic content was performed by colorimetric analysis. The
ethanolic extract was assessed for the antioxidant activity using three in vitro assay models, the 2,2-diphenyl-1-picrylhydrazylhydrate
(DPPH) radical scavenging assay, silver nanoparticles-based assay (SNPs), and an electron paramagnetic
resonance (EPR) radicals’ detection. SNPs method was described here for the first time in the C. parthenium extract. Using
high performance liquid chromatography - mass spectrometry (HPLC-MS) analysis, phenolic acid derivatives and free
aglycones of flavonoids were detected. The extract of C. parthenium with medium total polyphenol content (3.48%) showed
a moderate antioxidant activity, as demonstrated by the three methods, and these results could serve as a scientific basis for
its therapeutic uses.

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