THE SIMULTANEOUS DETERMINATION OF CANDESARTAN,
AMLODIPINE AND HYDROCHLOROTHIAZIDE BY
HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY, FROM A
MIXTURE AND PHARMACEUTICAL FORMULATIONS
ANCA MOISEI1, MARIA TOTAN1*, FELICIA GABRIELA GLIGOR1, ISABELA CRĂCIUN2, NICOLETA TODORAN3, AURELIA ADRIANA CHIŞ1, DANIELA ELENA POPA4
1“Lucian Blaga” University of Sibiu, Faculty of Medicine, Preclinical Department, 2A Lucian Blaga Street, 550169, Sibiu,
Romania
2Research and Development Centre, Polisano Pharmaceuticals SRL, 156 Alba Iulia Avenue, 550052, Sibiu, Romania
3University of Medicine and Pharmacy of Târgu-Mureș, Faculty of Pharmacy, Pharmaceutical Technology Department, 38
Gheorghe Marinescu Street, 540139, Târgu Mureș, Romania
4“Carol Davila” University of Medicine and Pharmacy, Faculty of Pharmacy, Drug Control Department, 6 Traian Vuia
Street, 020956, Bucharest, Romania
*corresponding author: bancioiumaria@yahoo.com
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Abstract:
The aim of this paper consisted in the development and validation of a high performance liquid chromatography (HPLC)
method for the determination of three antihypertensive substances from a mixture and pharmaceutical formulations. The three
selected antihypertensive substances were candesartan, amlodipine and hydrochlorothiazide. The extraction and
quantification of the constituents by HPLC was carried out using an analytical column, C18, and a mobile phase consisting in
a mixture of buffer solution pH = 3.5 and methanol (15:85 v/v), at a flow rate of 1 mL/ min. The UV detection was
performed at 240 nm for candesartan and amlodipine and at 270 nm for hydrochlorothiazide. The three compounds
mentioned above were separated with good resolution, reproducibility and sensitivity under these conditions. The proposed
HPLC method was applied to the analysis of the in-house dosage forms.

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