PHYTOCHEMICAL ANALYSIS OF HYPERICUM MACULATUM IN
ORDER TO OBTAIN STANDARDIZED EXTRACTS
ILIOARA ONIGA, ANCA TOIU*, DANIELA BENEDEC, IOAN TOMUŢĂ, LAURIAN VLASE
Faculty of Pharmacy, “Iuliu Haţieganu” University of Medicine and Pharmacy, 12, I. Creangă Street, 400010, Cluj-Napoca,
Romania
*corresponding author: ancamaria_toiu@yahoo.com
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Abstract:
The aim of this study was to perform a phytochemical analysis of Hypericum maculatum aerial parts harvested from different
areas of Transylvania (Romania) and to obtain a dry standardized and concentrated extract which can be easily used to
prepare tablets or capsules. Quantitative analyses of total flavonoids and hypericins for five samples of natural product were
performed by spectrophotometric methods, whilst the identification and quantitation of polyphenolic compounds by
HPLC/MS. The concentrations of flavonoids (3.7 - 5.5%) and total hypericins (0.12 - 0.74%) were determined. Caftaric acid,
chlorogenic acid, hyperoside, isoquercitrin, rutin, quercitrin and quercetin were identified and quantified by HPLC/MS. The
physicochemical characterization of the dry extract showed a similar content in active compounds as the ethanolic extract
from aerial parts.






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