DEVELOPMENT AND VALIDATION OF A NEW METHOD BY HIGH
PERFORMANCE LIQUID CHROMATOGRAPHY FOR THE
QUANTITATIVE ANALYSIS OF MAGNOLOL LOADED IN SILICA
PARTICULATE SYSTEMS
ALINA ȘTEFANACHE1, LĂCRĂMIOARA OCHIUZ1*, MARIA IGNAT2, ANDREEA CREȚEANU1, GLADIOLA ȚÂNTARU1
1”Grigore T. Popa” University of Medicine and Pharmacy Iași, 16 Universității Street, 700115, Iași, Romania
2“Al. I. Cuza" University, Faculty of Chemistry, 11 Carol I Bvd., 700506, Iași, Romania
*corresponding author: ochiuzd@yahoo.com
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Abstract:
In order to analyse magnolol (MG) in modern pharmaceutical forms, we developed and validated a new high performance
liquid chromatography (HPLC) method with UV detection. We optimized the working conditions for the chromatographic
system and recorded the chromatograms at the wavelength of 290 nm. The method was validated by the determination of the
following parameters: method repeatability and intermediate precision for the concentration of 0.5 mg/mL for which we
obtained RSD values of ≤ 2.5 for peak area, retention time and concentration, method linearity in the concentration range
0.025 - 1.5 mg/mL; calibration curve equation for MG (y = 492.05x + 6.5518) (R2 = 0.9985); detection limit of 0.00988
mg/mL and limit of quantification of 0.02993 mg / mL; method robustness for MG concentration of 0.5 to 1 mg/mL;
accuracy of method, for which the achieved recovery rate was in the range 98.42 - 103.83%. The method was tested by the
determination of MG loaded in nanoporous silica. Results showed a 96.57% degree of retrieval and confirm the hypothesis
that this method can be used for the quantitative determination of MG loaded in silicate matrices as modified release
formulations.

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