LC/UV/MS PROFILE OF POLYPHENOLS, ANTIOXIDANT AND
ANTIMICROBIAL EFFECTS OF AJUGA GENEVENSIS L. EXTRACTS
ANCA TOIU1, LAURIAN VLASE2, ANDREEA LETIȚIA ARSENE3*, DAN CRISTIAN VODNAR4, ILIOARA ONIGA1
1Department of Pharmacognosy, Faculty of Pharmacy, “Iuliu Hatieganu” University of Medicine and Pharmacy, 8, V. Babes
Street, Cluj-Napoca, Romania
2Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Pharmacy, “Iuliu Hatieganu” University of
Medicine and Pharmacy, 8, V. Babes Street, Cluj-Napoca, Romania
3Department of Biochemistry, Faculty of Pharmacy, “Carol Davila” University of Medicine and Pharmacy, 6, Traian Vuia
Street, sector 2, Bucharest, Romania
4Department of Food Science, Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary
Medicine, 3-5, Manăştur Street, Cluj-Napoca, Romania
*corresponding author: andreeanitulescu@hotmail.com
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Abstract:
The paper evaluated the polyphenolic content, antioxidant and antibacterial activities of Ajuga genevensis flower extracts.
Total phenolic content, total flavonoid content and HPLC/UV/MS were used for the determination and quantification of
polyphenols. Ethanol extracts were the richest in total phenols and flavonoids. Caffeic, p-coumaric and ferulic acids,
hyperoside, quercitrin, luteolin and apigenin were identified by LC/MS in all samples. The DPPH assay was used to evaluate
the antioxidant effects, and the results showed a better antioxidant activity for A. genevensis ethanol extract and a positive
correlation between antioxidant effect and polyphenolic content. Antimicrobial activity was tested using dilution assays,
minimal inhibitory concentration and minimal bactericidal concentration values were determined. The present study revealed
that A. genevensis flowers contain bioactive compounds with good antioxidant and antibacterial properties for pharmaceutical
and nutraceutical applications.

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