GRAPHENE OXIDE MODIFIED APTASENSOR FOR THE MUCIN 1
DETECTION
BIANCA CIUI1, MIHAELA TERTIŞ1,2, DIANA PEIA1, CECILIA CRISTEA1, ROBERT SĂNDULESCU1*
1”Iuliu Hațieganu” University of Medicine and Pharmacy, Faculty of Pharmacy,
Analytical Chemistry Department, 4 Pasteur Street, Cluj-Napoca, Romania
2”Ion Chiricuţă” Oncology Institute, 34-36 Republicii Street, Cluj-Napoca, Romania
*corresponding author: rsandulescu@umfcluj.ro
Download Full Article

Abstract:
An electrochemical aptasensor based on screen printed electrodes modified with graphene oxide was developed for the
indirect detection of Mucin 1 which is a tumour biomarker. The signal was obtained after the methylene blue electrochemical
reduction. The nanostructured platform was obtained via layer-by-layer deposition of graphene oxide onto the electrode.
After the complete optimization and characterization, the platform was incubated with the specific aptamer
(5´-GCAGTTGATCTTTGGATACCCTGGTTTTTTTTTTTTTTT-3´) with NH2 terminal group, and then the methylene blue
was used as redox probe in order to obtain the electrochemical signal. After the incubation with Mucin1, the aptasensor was
successfully tested using electrochemical impedance spectroscopy and differential pulse voltammetry.

Top