STUDY OF OFLOXACIN - RANDOM BY
METHYLATED - β - CYCLODEXTRIN INCLUSION COMPLEX
BLANKA SZÉKELY-SZENTMIKLÓSI1*, BÉLA TŐKÉS2
Faculty of Pharmacy, University of Medicine and Pharmacy of Târgu Mureş, Romania
1Department of Pharmaceutical Chemistry
2Department of Physical-Chemistry
*corresponding author: blanka.szekely@umftgm.ro
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Abstract:
Guest-host interactions of ofloxacin with randomly methylated beta-cyclodextrin (RAMEB) were assessed by
complementary analytical techniques. Computer assisted molecular modelling (ChemBio3DUltra 12.0) was utilized for
enantiomer-specific characterization of the complex. Ofloxacin - RAMEB complex was prepared by the kneading method
and the formation of inclusion complex was confirmed by IR spectroscopy. Differences in their affinity to host molecules
resulted in separation of the two enantiomers, thus capillary electrophoresis (CE) proved to be an eligible method for the
chiral separation of ofloxacin. The best separation was achieved using a 50 mM phosphate buffer, at pH 3.1, applying a
voltage of 20 kV at a temperature of 20°C and 40 mM RAMEB as chiral selector added to the background electrolyte. Under
these experimental conditions the chiral separation occurred in 6 minutes.






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