A NEW NATURAL ANTIOXIDANT SUPPLEMENT – DESIGN AND
DEVELOPMENT
TÜNDE JURCA1, LAURA VICAS1*, ELEONORA MARIAN1, SIMONA VICAS2*, MARIANA MURESAN1
1University of Oradea, Faculty of Medicine and Pharmacy, Department of Pharmacy, Oradea,, Romania
2University of Oradea, Faculty of Environmental Protection, Department of Biochemistry, Oradea,, Romania
*corresponding author: laura.vicas@gmail.com
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Abstract:
Various preparations based on herbs, when taken in sufficient doses and correct combinations, neutralize free radicals before
they lead to the appearance of diseases in the body. In order to obtain a new nutritional supplement, we selected raw vegetal
products: blueberry fruits (Vaccinium fructus), seabuckthorn fruits (Hippophaë Rhamnoides fructus), sage leaves (Salviae
folium) and marigold flowers (Calendulae flos). The parts of plants originate from spontaneous flora. The pharmaceutical
form of the final product is represented by antioxidant capsules. The antioxidant activity of the final product was
demonstrated by the quantification of polyphenols (Folin-Ciocâlteu method), flavonoids (colorimetric method) of
anthocyanins (spectrophotometric UV-VIS method). It has been also determined the content of heavy metals, elements that
are good catalysts for biochemical processes. In vitro dissolution test is a quality parameter, in order to assess the
bioavailability of the active ingredients in this pharmaceutical form achieved. The evaluation of the in vitro release of the
active ingredients in the capsules under compendial conditions was performed on a number of 3 capsules, for 45 minutes
according to the Romanian Pharmacopoeia 10th edition. The profile of the in vitro release of the active substances was carried
out using 6 points, corresponding to the amount of active ingredients released at 5, 10, 15, 20, 30 and 45 minutes. The
evaluation of antioxidant activity of the released active ingredients was performed by the α,α-diphenyl-β-picrylhydrazyl free
radical scavenging method (DPPH). It has been also carried out the evaluation of microbial load of required parts of plants in
order to reduce the risk of an onset of infections.






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