VIDEO RECORDING AND TIME RESOLVED DIGITAL PARTICLE
IMAGE VELOCIMETRY IN EVALUATION OF ANTIAGGREGANT
DRUGS
IULIAN SARBU, ERAND MATI, STILIYANA BORISOVA, ALICE PIPEREA-SIANU, IRINA PRASACU*
“Carol Davila” University of Medicine and Pharmacy, Faculty of Pharmacy, Doctoral School, Bucharest, Romania
*corresponding author: irinaprasacu@yahoo.com
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Abstract:
This paper presents an analysis of erythrocytes aggregation and sedimentation processes, using three methods: (i) the
sedimentation curve determined by measuring the height of sediment, (ii) the video observation and recording of microscopic
images during sedimentation (iii) the analysis of digital particle image velocimetry (PIV) as mathematical method for the
estimation of red blood cells aggregates trajectories and velocities. The main finding was the co-existence of ascendant and
descendent flow tubes. Single erythrocytes and small aggregates appeared to move up as a counter stream coupled with
descending flow of greater aggregates. The mathematical analysis revealed an even more complex picture, including
turbulences and formation of local vortexes. Since our experiments with clopidogrel proved an effect of decreasing
erythrocytes sedimentation, the PIV method was further applied in analysis of samples containing 4 μg/mL drug. It was
observed a significant change in the distribution of speed velocities.

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