CHIRAL SEPARATION OF 16 BETA-BLOCKERS ON IMMOBILIZED
POLYSACCHARIDE CHIRAL STATIONARY PHASES
RADU-CRISTIAN MOLDOVAN1, GABRIEL-SORIN DASCĂL1, VALENTIN MIREL2, EDE
BODOKI1*, RADU OPREAN1
1Department of Analytical Chemistry and Instrumental Analysis, Faculty of Pharmacy, “Iuliu Hațieganu” University of
Medicine and Pharmacy Cluj-Napoca, 4 Louis Pasteur Street, 400349 Cluj-Napoca, Romania
2National Institute for Research and Development of Isotopic and Molecular Technologies, 67-103 Donat Street, 400293
Cluj-Napoca, Romania
*corresponding author: bodokie@umfcluj.ro
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Abstract:
Chiral separations method development is a tedious process, involving the use of high quantities of solvents and it is aquired
over a long period of time. In order to simplify the process and reduce the time needed for selecting the optimal stationary
phase for separating the enantiomers of interest, a normal phase gradient elution was developed using n-hexane/2-propanol as
mobile phase with an alcohol gradient ranging from 20% to 50% (v/v). Four commercially available polysaccharide based
stationary phases were tested on a set of 16 chiral beta-blockers. The effect of three basic additives was evaluated regarding
their effect on selectivity. It was observed that depending on the stationary phase, the additives can have an important
influence. This approach can supply a convenient set of data for future quantitative correlations between chemical structure
of enantiomers and experimental chromatographic parameters. Moreover, the optimal mobile phase composition can be
chosen for further development in isocratic mode.

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