IN VITRO MODEL FOR TOTAL ANTIOXIDANT ACTIVITY
EVALUATION WITH APPLICATIONS ON BIOLOGICAL PRODUCTS
ALINA MONICA MIFTODE1, ALINA ŞTEFANACHE1*, ADRIAN ŞPAC2, VASILE DORNEANU3
“Gr. T. Popa” University of Medicine and Pharmacy, Faculty of Pharmacy, 16 Universităţii Street, 700115 Iaşi, Romania
1Inorganic Chemistry Department;
2Physical Chemistry Department;
3Analytical Chemistry Department.
*corresponding author: stef.alina@yahoo.com
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Abstract:
In the present paper we are proposing an in vitro model for assessing the overall antioxidant activity by using the radical
cation chromophore of chlorpromazine (CP•+) which presents a maximum absorption at 525 nm. A decrease in the radical
colour is caused by the presence of antioxidants, proportional to the concentration of antioxidant. For evaluating the
antioxidant action, the percentage inhibition of absorbance is being calculated for two established time moments (10 and 20
min), then the kinetic graph is drawn (percentage inhibition as a function of the time), and the area under curve (AUC) is
calculated. From the calibration curve, found under similar conditions, (the AUC as a function of the standard antioxidant
concentration - gallic acid) one may determine the total antioxidant activity expressed in gallic acid equivalents (GAE). The
calibration curve was plotted on two concentrations intervals: 10-50 μM/L and 50-100 μM/L of gallic acid. The method we
are proposing involves both the concentration of the antioxidant as well as the time of reaction, constituting a thorough
measure of the antioxidant’s ability to scavenge free radicals, related to a standard antioxidant, at selected time points of the
reaction. The model may be applied on determining the antioxidant action of complex composition biological products.






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