IN VITRO/IN VIVO PERFORMANCE STUDY OF NEW
METRONIDAZOLE PERIODONTAL GEL FORMULATIONS
ADRIANA CIURBA1, LUMINIȚA LAZĂR2*, PAULA ANTONOAEA1, ANCA MEDA
GEORGESCU3, CAMIL-EUGEN VARI4, NICOLETA TODORAN1
University of Medicine and Pharmacy of Tîrgu Mureş, Romania
1 Department of Pharmaceutical Technology, Faculty of Pharmacy
2 Department of Periodontology, Faculty of Dental Medicine
3 Department of Infectious Diseases, Faculty of Medicine
4 Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy
*corresponding author: luminita.lazar@umftgm.ro
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Abstract:

The aim of this paper is to continue previous studies of gel formulation containing metronidazole and having mucoadhesive
properties for the treatment of periodontal diseases by assessing their in vitro and in vivo characteristics. We started from the
premise that the crosslink density and swelling capacity of the polyacrylic acid derivatives greatly affect the swelled
mucoadhesive polymer which penetrates into the tissue crevices followed by the interpenetration between the polymer chains
and protein chains of the mucus aimed to achieve correlations between the rheological properties of gels and mucoadhesivity.
The results showed that the analysed gels F3_3, F3_1 (Ultrez 10) and F2_3 (Carbopol® 974P) have the most appropriate
rheological properties. To evaluate the kinetics of drug release from the analysed gels, 9 kinetic models (functions of time)
were performed. R2
adjusted (the adjusted coefficient of determination), MSC (model selection criterion) and AIC criterion
(Akaike informational criterion) used as goodness of fit parameters indicated that Korsmeyer-Peppas is the most appropriate
model to describe the release kinetics of the drug release from the 9 gel formulations. Preliminary clinical studies have shown
that the topical application of metronidazole gel formulations, in combination with mechanical treatment of smoothening of
the root surface appears to be more efficient, improving clinical parameters. Plaque index (PI), bleeding on probing index
(BOP), and probing pocket depth (PPD) evaluated at one month post-treatment had better values compared with those of the
initial examination values in a greater number of the periodontal examined sites (PI-54.15%, BOP-62.38%, and PPD-
28.45%) in the group B of patients receiving scaling and root planning (SRP) and 15% metronidazole gel formulation F3_3.






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