EFFECT OF ANTIBIOTIC TREATMENT ON TGF-Β1 EXPRESSION IN
GINGIVAL TISSUES OF PATIENTS WITH CHRONIC PERIODONTAL
DISEASE
MARILENA BĂTĂIOSU1#, ANCA PREDESCU2#, MONICA SCRIECIU2*, MIHAELA ŢUCULINĂ1,
CONSTANTIN DĂGUCI1, LUMINIŢA DĂGUCI1, ANA MARINA ANDREI3, IULIANA CEAUȘU4,
ILEANA MONICA BANIŢĂ1, CĂTĂLINA GABRIELA PISOSCHI3
1Faculty of Dentistry, University of Medicine and Pharmacy, 2 Petru Rareş Street, 200349, Craiova, Romania
2Faculty of Medicine, University of Medicine and Pharmacy, 2 Petru Rareş Street, 200349, Craiova, Romania
3Faculty of Pharmacy, University of Medicine and Pharmacy, 2 Petru Rareş Street, 200349, Craiova, Romania
4Faculty of Medicine, University of Medicine and Pharmacy “Carol Davila”, Department of Obstetrics and Gynaecology,
“Dr. I. Cantacuzino” Hospital, 5-7 Ion Movila, Bucharest, Romania
*corresponding author: scrieciu_monica@yahoo.com
#Authors have equal contribution to this paper.
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Abstract:

Chronic periodontitis is initiated by subgingival biofilm depots that induce a release of a cascade of inflammatory molecules
in periodontal structures which finally lead to the destruction of the supporting tissues and alveolar bone. One of these
cytokines is the transforming growth factor-β1 (TGF-β1) associated with inflammatory host response, but also involved in
healing and fibrosis. Correlations between periodontal tissues degradation and TGF-β1 expression have been intensively
investigated, but the studies regarding modulation of TGF-β1 expression under systemic antibiotic therapy are scarce. In this
study we assessed the effect of two combinations of antibiotics used to treat chronic periodontitis on the relation between
TGF-β1 expression and the indexes used for clinical evaluation of periodontium destruction. Gingival samples collected from
patients with untreated chronic periodontitis and those receiving either the combination amoxicillin-metronidazole or
spiramycin-metronidazole were processed for paraffin embedding and then stained for routine histological examination and
immunohistochemistry in order to assess the morphological changes and to detect the localization of TGF-β1. TGF-β1
displayed different patterns of expression between the groups included in the study, being weaker and scarce in samples from
patients treated with antibiotics in contrast with untreated subjects. The clinical index was in accordance with
immunohistochemical results. As a conclusion, TGF-β1 may have a contribution not only to the pathogenesis of periodontal
disease but also in the healing mechanism by means of antibiotic treatment.

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