RESEARCH REGARDING TARAXACUM
OFFICINALE (L.) WEBER
II. OPTIMUM EXTRACTION PARAMETRES OF
CAFFEIC ACID DERIVATIVES USING
ULTRASOUND - ASSISTED EXTRACTION
NATELA STYLIANOU1, VASSILIS GEKAS2, ADRIANA IULIANA
ANGHEL3*, VIORICA ISTUDOR1
1University of Medicine and Pharmacy “Carol Davila”, Faculty of
Pharmacy, 6 Traian Vuia, 020956, Bucharest, Romania
2Cyprus University of Technology, Department of Agricultural Sciences,
Biotechnology and Food Science, Lemesos 3603, Cyprus
3University of Medicine and Pharmacy “Carol Davila”, Faculty of
Pharmacy, Department of Pharmaceutical Botany, 6 Traian Vuia,
020956, Bucharest, Romania
*corresponding author: anghel_adriana_iuliana@yahoo.com
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Abstract:

Pharmacological studies confirmed the therapeutic properties of the phenolic
acids from plants, and their efficiency in treatment of type 2 diabetes mellitus. Based on
this and also, on the facts that Taraxacum officinale L. contains a considerable amount of
caffeic acid derivatives (48.8 mg/g in the leaves) and it does not exhibit a cytotoxic activity,
we aimed to obtain a pharmacological active dry extract, titrated in cichoric acid, which can
be developed in a pharmaceutical product used in type 2 diabetes mellitus therapy.
The aim of this work was to optimize and evaluate the extraction parameters of
caffeic acid derivatives from the plant material using Ultrasound - Assisted Extraction
(UAE). The effects of different parameters of caffeic acid derivatives yields, in plants
extracts were investigated using an experimental design - Taguchi L16 (45) orthogonal array
design. An HPLC/UV method was employed for the qualitative and quantitative
determination of caffeic acid derivatives from the liquid extracts and from the lyophilized
extract. The amount of caffeic acid derivatives and cichoric acid in the dry extract was
127.0 mg/g and 107.50 mg/g.

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