MONITORING OF SKIN PENETRATION AND
ABSORPTION WITH A NEW IN VIVO
EXPERIMENTAL MODEL
SZILVIA BERKÓ1, BOGLÁRKA BALÁZS1, BLANKA SÜTŐ1,
GÁBOR ERŐS2, BRIGITTA GÁL2, ANITA SZTOJKOV-IVANOV3,
CODRUȚA SOICA4, PIROSKA SZABÓ-RÉVÉSZ1, LAJOS KEMÉNY2,
ISTVÁN ZUPKÓ3, ERZSÉBET CSÁNYI1*
1Department of Pharmaceutical Technology, University of Szeged, 6720
Szeged, Eötvös street 6., Hungary
2Department of Dermatology and Allergology, University of Szeged, 6720
Szeged, Korányi street 6-8., Hungary
3Department of Pharmacodynamics and Biopharmacy, University of
Szeged, 6720 Szeged, Eötvös street 6., Hungary
4Department of Pharmaceutical Chemistry, University of Medicine and
Pharmacy "Victor Babes" Timisoara, 300041 Timisoara, 2 Eftimie
Murgu Str., Romania
*corresponding author: csanyi@pharm.u-szeged.hu
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Abstract:

The purpose of the study was to use a new type of in vivo animal model
developed by our research group for the investigation of skin penetration, compared to the
results of Franz cell measurements. The new in vivo experimental model provides
possibility for the exact measurement of the quantity of the penetrated drug through the
living mouse skin. Two gel formulations containing ibuprofen were investigated with or
without penetration enhancer. The applied penetration enhancer was Transcutol®. The
effect of the penetration enhancer was clearly detected in both models. The in vitro diffused
amount of ibuprofen was significantly higher than the one permeated through the living skin.
The presence of the active agent in the plasma was also determined after the observation period.
It was possible to monitor the penetration and absorption of topical formulations simultaneously.

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