RESEARCHES UPON INDIGENOUS HERBAL
PRODUCTS FOR THERAPEUTIC
VALORIFICATION IN METABOLIC DISEASES.
NOTE II. POLYPHENOLS CONTENT,
ANTIOXIDANT ACTIVITY AND
CYTOPROTECTIVE EFFECT OF BETULAE
FOLIUM DRY EXTRACT
TEODORA COSTEA1, LAURIAN VLASE2*, VIORICA ISTUDOR1,
MARIA-LIDIA POPESCU1, CERASELA-ELENA GÎRD1
1University of Medicine and Pharmacy ,”Carol Davila”, Faculty of
Pharmacy, Pharmacognosy, Phytochemistry, Phytotherapy Department,
6 Traian Vuia Street, 020956, Bucharest,
2University of Medicine and Pharmacy ,”Iuliu Haţieganu“, Faculty of
Pharmacy, Department of Pharmaceutical Technology and
Biopharmaceutics, 12 I. Creangă Street, 400023, Cluj-Napoca, Cluj
*corresponding author: vlaselaur@yahoo.com
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Abstract:

Among natural compounds, polyphenols are well-known antioxidants with
beneficial effects towards endothelial dysfunction, the main cause of vascular
complications in type 2 diabetes mellitus (DM 2). In the present study, a Betulae folium dry
extract was evaluated for its polyphenols content, antioxidant activity and protective effect
against OH• free radicals (generated by H2O2 and FeSO4 reaction) induced oxidative stress
in EA.hy926 endothelial human cell line. Spectrophotometric and LC-MS analysis were
used for polyphenols determination, 2,2-diphenyl-1-picrylhidrazyl free radical (DPPH) for
antioxidant activity and cellular viability (MTT assay) for evaluating the cytoprotective
effect. Betulae folium dry extract is a rich source of polyphenols, with antioxidant activity
(IC50 = 0.1841mg/mL) and has a modest cytoprotective effect in a narrow concentration
range (0.03-0.05 mg/mL).

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