EFFECT OF THREE MONOGLYCERIDE BASED
CUBOSOMES SYSTEMS ON THE VIABILITY OF
HUMAN KERATINOCYTES
ANDRA TUDOSE1, CHRISTIAN CELIA2, 3, IONELA BELU4*,
STILIYANA BORISOVA1, DONATELLA PAOLINO2
1University of Medicine and Pharmacy “Carol Davila” Bucharest,
Faculty of Pharmacy,
2Department Pharmacy, University “G. d’Annunzio” of Chieti – Pescara,
Via dei Vestini 31, 66013, Chieti (CH), Italy.
3The Methodist Hospital Research Institute, Department of
Nanomedicine, 6670 Bertner Avenue, 77030 Houston, TX, USA.
4 Craiova University of Medicine and Pharmacy, Craiova, Romania
* corresponding author: ionela_d@yahoo.com
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Abstract:

Three lipid-based cubic liquid crystalline nanoparticles (or cubosomes) systems were formulated and prepared by emulsifying surfactants Brij® 30 and Brij®35 with aqueous solution of poly(ethylene) glycol derivatives and glyceryl monooleate: one formulation containing Monomuls® 90-O18 and Pluronic® PE 10500, another one containing Monomuls® 90-O18 and Brij®35, and the third one based on Pluronic® PE 6800, Brij®30 and Brij®35. Toxicity of formulations was evaluated by measuring the effect of
those cubosomes on human keratinocytes (NCTC2544) viability. Cells were seeded at densities of 15,000 and 30,000 cells per well, respectively, into a 24-well tissue culture dish. The cell viability test was performed using 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay by measuring oxidative activity of mitochondrial succinate dehydrogenase enzyme. Effect on cell viability for each formulation was determined as function v(t, c) of time (24, 48 and 72 hours) and cubosomes concentrations (0, 5, 10, 20, 25 and 50 μg/mL)....

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