SPECTROPHOTOMETRIC METHOD FOR
LISINOPRIL DETERMINATION USING
NINHYDRIN
LAURA SBÂRCEA1*, LUCREŢIA UDRESCU2, LIANA DRĂGAN1,
CRISTINA TRANDAFIRESCU1, ZOLTÁN SZABADAI2, MARIUS
BOJIŢĂ3
“Victor Babeş” University of Medicine and Pharmacy, Faculty of
Pharmacy, 2 Eftimie Murgu Square, 300041, Timişoara, Romania
1 Departament of Pharmaceutical Chemistry
2 Departament of Drug Analysis
3 “Iuliu Haţieganu” University of Medicine and Pharmacy, Faculty of
Pharmacy, Departament of Drug Analysis, 6 Louis Pasteur St., 400015,
Cluj-Napoca, Romania
*corresponding author: laura_sbarcea@yahoo.com
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Abstract:

This paper presents a sensitive, accurate and rapid method for quantitative
determination of lisinopril dihidrate (LIS) in bulk and pharmaceutical formulations based on the reaction with ninhydrin in the presence of potassium hydroxide. The reaction quantitatively proceeds at a temperature of 95 ± 2ºC, in 10 min and the end product, purple coloured, exhibits maximum absorption at 567 nm. The Lambert-Beer law is obayed within
the range of 10 – 30 μg/mL, while the calibration curve is described by the regression equation A = 0.0312 C (μg/mL) – 0.2262, having a correlation coefficient of R2 = 0.9981 (n = 8). The influence of temperature, heating time and reactive concentrations on the end product was also analyzed, so as to render the optimal conditions for reaction. In order to validate the method, the linearity, the limit of detection (LOD), limit of quantification
(LOQ), sensitivity, selectivity, precision, accuracy and recovery were determined according to ICH guidelines.






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