DETERMINATION OF ASCORBIC ACID IN
SHOOTS FROM DIFFERENT CONIFEROUS
SPECIES BY HPLC
VALERIA RADULESCU1, DIANA-CAROLINA ILIES*1, ION
VOICULESCU2, IOVU-ADRIAN BIRIŞ2, ADAM CRACIUNESCU3
1Department of Organic Chemistry, Faculty of Pharmacy, University of
Medicine and Pharmacy “Carol Davila”, 6 Traian Vuia Street, 020956
Bucharest, Romania
2Forest Research and Management Institute, 128 Eroilor Blv., 077190 Voluntari,
Ilfov, Romania
3National Forest Administration-Romsilva, 9A Petricani Street, 010325
Bucharest, Romania
*corresponding author: ilies_diana@hotmail.com
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Abstract:

The stability of ascorbic acid (AA) in different aqueous solutions was studied for the development of optimal methods and procedures for the HPLC analysis of this compound in young shoots with needles of conifers. The best extraction solvent that offered the maximum stability of AA was aqueous 0.5% acid oxalic. The chromatographic separation was carried out on Lichrosorb RP-18 column using as mobile phase: 95% NaH2PO4 0.05 M (aqueous solution) adjusted to pH 3.6 and 5% methanol. The method proposed was applied to the analysis of AA in shoots samples of five coniferous species: silver fir (Abies alba Mill.), Douglas-fir (Pseudotsuga menziesii (Mirbel) Franco), European larch (Larix decidua Mill. ssp. carpathica (Dom.) Siman), Norway spruce (Picea abies L. Karst.), and black pine (Pinus nigra Arn. ssp. nigra).

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