SUPEROXIDE DISMUTASE LOADED
LIPOSOMES. THE INFLUENCE OF
FORMULATION FACTORS ON ENZYME
ENCAPSULATION AND RELEASE
ALINA SILVIA PORFIRE, IOAN TOMUŢĂ*, SORIN EMILIAN
LEUCUŢA, MARCELA ACHIM
Department of Pharmaceutical Technology and Biopharmaceutics,
Faculty of Pharmacy, “Iuliu Hatieganu” University, 400023, Cluj-
Napoca, Romania
*corresponding author: tomutaioan@yahoo.com
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Abstract:

The objective of this paper was to prepare superoxide dismutase (SOD) loaded conventional and PEG-coated (polyethylene glycol) liposomes and to study the influence of some formulation factors on their characteristics in terms of facilitating antioxidant potential and delivery. An experimental design with 3 factors and 2 levels was used, in order to optimize SOD encapsulation efficiency and to determine the activity of released
SOD in certain experimental conditions. The studied variables were: the molar ratio phospholipids:cholesterol, the type of phospholipids used and the concentration of SOD solution used for liposomes preparation. The liposomes were characterized through SOD concentration, encapsulation efficiency, % of SOD released after 24h at 4°C and % of SOD released after 24h at 37°C. The results showed that the concentration of SOD solution used in the preparation process had a significant effect on all studied responses, making this drug delivery systems promising for reliable and safe therapies.






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